당도, pH 측정 발효액을 cheese cloth를 이용하여 여

당도, pH 측정
발효액을 cheese cloth를 이용하여 여과한 후 원심분리 기(Centrifuge 5810R, Eppendorf, Hamburg, Germany)를 이용하여 3,QQQXg, 10분간 원심분리 한 상등액을 시료로 하 여 당도는 Master Refractometer(N-lE, ATAGO, Tokyo, Japan)를 이용하였고, pH는 pH meter(Orion 410A, Orion Research Inc., Boston, MA, USA)를 사용하여 측정하였댜
미생물수 측정
미생물수는 일정기간별로 시료를 채취하여 0.1% 펩톤수 로 적 정 희 석 한 후 총 균수는 plate count agar(PCA, Difeo, Detroit, MI, USA), 효모와 곰팡이 수는 potato dextrose agar(PDA, Difeo), 대장균군수는 violet red bile agar (VRBA, Difeo)에 접종하여 37°C에서 24시간 배양한 후 나 타난 colony를 계수하여 CFU(colony forming unit)/mL로 나타내었다.
효소 활성 측정
발효액을 원심분리(3,000xg, 10 min) 하여 상등액 2 mL 에 메탄올을 가하여 10 mL로 정용하고 균질화한 후 이를 다시 원심분리 하여 침전물에 정제수를 가하여 용해시킨 다 음 원심분리 한 상등액을 효소 활성 측정용 시료로 사용하였 다(2). Amylase는 1% soluble starch(in 50 mM phos¬phate buffer, pH 6.9)를 기질로 사용하여 40°c에서 30분 간 사용하였고, invertase와 cellulase는 1% sucrose(in 50 mM sodium acetate buffer, pH 4.6)와 0.5% carboxy¬methyl cellulose(CMC, in 50 mM sodium acetate buffer, pH 5.0)를 각각 기 질로 사용하여 40°c에 서 30분간 반응시 켰댜 이때 반응액의 효소 활성은 glucose(Sigma-Aldrich Co., St. Louis, MO, USA)를 표준품으로 하여 환원당 함량 을 측정한 후 활성을 산출하였으며, 효소 단위는 1분간 1 µg의 glucose를 생성하는 효소의 양을 1 unit으로 하였다 (7).
폴리페놀 함량 측정
95% 에 탄올(Duksan Pure Chemical Co., Ansan, Ko¬rea)로 100배 희석한 후 원심분리(3,000Xg, 10 min) 및 여 과(No. 2, Whatman International Ltd., Leicestershire, UK)하여 시료로 사용하였댜 총 폴리페놀 함량 측정은 Folin-Denis법 (8)에 따라 시 료 1 mL에 0.2 N Falin¬Ciocalteu's phenol reagent(Sigma-Aldrich Co.) 1 mL를 가하여 실온에서 3분간 반응시킨 후, 7.5% Na2C03 1 mL를 가한 뒤 암소에서 1시간 동안 방치한 다음 spectropho¬tometer(Ultraspec 1000, Pharmacia Biotech, Cam-

結果 (英文) 1:

Brix, pH measurement Fermentation liquid using cheese cloth filtration after centrifugation (Eppendorf, Hamburg, Germany 5810R, Centrifuge) 3, QQQXg, and 10 minutes centrifugation to sample, such as a prize by the Master sugar Refractometer (N-lE, ATAGO, Tokyo, Japan), and pH of the pH meter (Orion Orion Research Inc., Boston, MA, 410A, USA) were measured using multiple Microbiological measurements The number of micro-organisms by 0.1% PEP by picking a sample schedule tonnage as ever, the number of total bacteria after Jung-Hee-Seok plate count agar (PCA, Difeo, Detroit, MI, USA), yeast and potato dextrose agar be fungus (PDA, Difeo) violet red bile agar, e. coli are munitions (VRBA, Difeo) at 37 ° C with dose of culture, 24 hours a day, and then I get I colony counting the CFU (colony forming unit)/mL as indicated. Enzyme activity measurements Fermented liquid centrifugation (3, 000xg, 10 min) to the master output 2 mL to 10 mL methanol to the Jung dragons and homogenization, and then again by centrifugation sediment in purified water to dissolve well done that by centrifugation to enzyme activity measurements such as a prize sample was used as (2). Soluble starch Amylase is 1% (in 50 mM pH buffer, phos phate ¬ 4.3) use the 40 ° c as a substrate in 30 minutes use, invertase and sucrose 1% cellulase (in 50 mM sodium acetate buffer, pH 4.6) and 0.5% carboxy methyl cellulose ¬ (sodium acetate buffer, CMC, in 50 mM pH 5.0) for each machine uses 40 ° c into the vagina as a 30-minute response to let multiple at this point, the reaction of the enzyme is glucose (Sigma-Aldrich Co. , St. Louis, MO, USA) won the party into the fold with standard content after measuring the output, active enzymes, one minute 1 µ g of glucose, the amount of the enzyme to generate 1 unit to (7). The polyphenol content measurement 95% come on (Duksan Pure Chemical Co., Ansan, Ko rea ') after centrifugation diluted 100 times (3, 000Xg, 10 min) and filtration (No. 2, Whatman International Ltd., Leicestershire, UK) was used as a sample by the total polyphenols content in multiple measures in accordance with the law (8): fee Folin-Denis 1 mL in 0.2 N Ciocalteu's phenol reagent Falin ¬ (Sigma-Aldrich Co.) 1 mL to 3 minutes at room temperature by reaction, 1 mL, 7.5% goes back in the cow Na2C03 hour-long neglect then spectropho ¬ tometer (Ultraspec 1000 , Pharmacia Biotech, Cam-

結果 (英文) 3:

the ph was measured.the entry into force of the cheese cloth, filtration, centrifugal separation, Centrifuge 5810 r, Eppendorf, Hamburg, Germany) the use of three QQQXg 10 minutes a fine in the amount of centrifugal separation treatment by the party is Master Refractometer (n - lE, ATAGO, Tokyo, Japan) use. the ph, the ph is meter (Orion 410 a Research Orion Inc. Boston, ma, USA) using the true test.determination of microbial numbermicroorganisms can be within a certain period of time don"t like data mining, 0.1% peptone water with the city a total of three, this is plate count agar (PCA, Difeo, Detroit, MI, USA), yeast and fungi can potato dextrose agar (pda, Difeo), the number of escherichia coli violet red bile agar (VRBA, Difeo vaccination, 37 degrees c) after 24 hours of culture in the colony, on the CFU (colony forming unit) / mL for display.enzyme activity measurementthe entry into force of the centrifugal separator (3000 xg 10 min), no. 2 in the mL, methanol is 정용 and homogeneous group with 10 mL after centrifugal separation, precipitation and dissolved in water and soil by the centrifugal separation of a higher amount of enzyme activity of the sample used. (2). Amylase soluble starch (1% in 50 mM phos ¬ phate buffer, ph 6.9) used as substrates, 40 degrees c in 30 minutes, and the use of 1%, invertase cellulase sucrose (in 50 mM sodium acetate buffer, ph 4.6) and 0.5% carboxy ¬ methyl cellulose (CMC, in in mM sodium acetate buffer, ph 5.0), can be used for the 40 degrees c, and the reaction time was 30 minutes, the true response of enzyme activity is glucose (Sigma - Aldrich Co. St. Louis, mo, USA) with the standard, the measurement of the content. calculation, mold units 1 µ grams of 1 minute to create glucose is one unit of the enzyme amount. (7).determination of phenol content.sitting in the back (95% Duksan Pure Chemical Co. Ansan, Ko ¬ rea) diluted 100 times (3000 Xg, centrifugal separation, and 10 min) and female (no 2, Whatman International Ltd. Leicestershire, UK), used for the treatment of the phenol content is true of Folin the Denis법 (8) in accordance with the time data in the first mL 0.2 n Falin ¬ Ciocalteu "s phenol reagent (Sigma - Aldrich Co mL) 1 to 3 minutes, the room temperature in a reaction by the Na2C, 7.5% have a mL after one hour, no noise in the spectropho ¬ tometer (Ultraspec 1000, Pharmacia Biotech, Cam -